General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 2pl Kit. (93-1061E3CP5M)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cellular Dynamics International iCell Cardiomyocytes, human iPSC-derived, 3 vials, ≥ 1.0 x 10^6 viable cells each, >95% cTNT-positive, cryopreserved single-cell suspension
iCell Cardiomyocytes, human iPSC-derived, 3 vials, ≥ 1.0 x 10^6 viable cells each, >95% cTNT-positive, cryopreserved single-cell suspension
AdipoGen IL-10 human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human IL-10 in serum, plasma and cell culture supernatant. Range 3.125 to 200pg/ml. Sensitivity 1.5pg/ml. Samples Cell Culture Supernatant, Plasma, Serum. The cytokine Interleukin-10 IL-10 is a key anti-inflammatory mediator. IL-10 limits the immune response to pathogens and microbiota and thereby prevents damage to the host while playing important roles in other settings such as sterile wound healing, autoimmunity, cancer and homeostasis. IL-10 is the founding member of a family of cytokines that also includes IL-19, IL-20, IL-22, IL-24, IL-26, IL-28A, IL-28B and IL-29. IL-10 is expressed by cells of both the myeloid and lymphoid lineages, including macrophages, monocytes, DCs, neutrophils, mast cells, eosinophils and natural killer cells, in addition to CD4 and CD8 T cells and B cells. IL-10 is also produced by resident macrophages, such as microglia and cardiac macrophages.
Meso Scale Discovery RPLEX HUM Osteoprotegerin ASY
The Meso Scale Discovery R-PLEX Human Osteoprotegerin Assay is a multiplex immunoassay panel that measures osteoprotegerin (OPG) in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This assay requires 50 L or less sample volume and can be fully automated on the MSD instrument platforms It is intended for research use only to study OPG levels and not for diagnostic procedures This assay measures total OPG and can aid in understanding bone remodeling processes
Eurofins Discoverx Calcium No WashPLUS, 10 plates (384-well). (90-0091)
The Calcium No WashPLUS detection kit is intended for use with cells expressing receptors that signal through calcium. The kit contains a calcium-sensitive dye which assays intracellular calcium levels upon activation of a Gαq-coupled GPCR. Activation of the GPCR leads to release of intracellular calcium, and a detectable increase in fluorescent signal. This detection kit contains reagents for assaying 10 plates. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/cell-line/calcium-no-washplus-90-0091.
Eurofins Discoverx PathHunter Darbepoetin Alfa Bioassay Kit, 10pl. (93-0965Y3-00020)
The PathHunter Darbepoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoetin or darbepoetin alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The cell line included overexpresses EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress C5AR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0777E3CP5L)
The PathHunter C5AR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of C5AR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged C5AR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting C5AR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Creative Biomart Fructose Assay Kit is a quantitative colorimetric fructose determination at 565 nm.
Direct Assays: fructose in biological samples (e.g. serum, plasma, urine, saliva, milk, culture medium), food, juice, beverage and other agricultural products.Drug Discovery/Pharmacology: effects of drugs on fructose metabolism.Storage : Store all components at -20°C. Shelf life of six months after receipt.
Enzo Life Sciences SOD activity kit (5x96wells)
ELS offers a large collection of superoxide dismutase products for oxidative stress research. This is a versatile assay for the determination of SOD activity. Differentiate between the activity of three SOD isoenzymes: MnSOD, FeSOD, and Cu/ZnSOD. Obtain higher throughput sample analysis with a convenient colorimetric 96 well plate format and fast results in just 10 minutes. Alternative Name: Superoxide dismutase. Applications: Colorimetric detection, Activity assay. Contents: Microtiter plate, Standard, SOD buffer concentrate, Xanthine oxidase, Xanthine solution, Triton x-100, WST-1 reagent
Abcam DNMT3B Assay Kit.
DNMT3B Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress EphB2 Functional Assay, 10-Plate Kit. (93-0848E3CP16L)
The PathHunter eXpress EphB2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB2 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1B U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0697E3CP6L)
The PathHunter HTR1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1B and EA-tagged β-Arrestin-2. Activation of HTR1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.