General Cell Based Assays
Eurofins Discoverx PathHunter eXpress mOPRD1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0611E2MCP0M)
The PathHunter mOPRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of mOPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRD1 and EA-tagged β-Arrestin-2. Activation of mOPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human AQ STING Binding Kit, 500 Assay Points
HTRF Human AQ STING Binding Kit, 500 Assay Points
Montana Molecular Human Adenosine A1 Receptor (AA1R, ADORA1) Big Sky BacMam, 15mL
Human A1 Adenosine receptor packaged in Montana Molecular's Big Sky BacMam vector. Express the adenosine receptor in your cells of interest. Big Sky BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.
Eurofins Discoverx PathHunter eXpress AVPR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0588E2CP0L)
The PathHunter AVPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR2 and EA-tagged β-Arrestin-2. Activation of AVPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mVIPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0619E2MCP2M)
The PathHunter mVIPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of mVIPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mVIPR1 and EA-tagged β-Arrestin-2. Activation of mVIPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0308E2CP2M)
The PathHunter SSTR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR4 and EA-tagged β-Arrestin-2. Activation of SSTR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mGHSR1a CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0605E2MCP2L)
The PathHunter mGHSR1a eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. grehlin) based activation of mGHSR1a GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGHSR1a and EA-tagged β-Arrestin-2. Activation of mGHSR1a stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 16 Reagent, 100. (93-0563R16A)
AssayComplete Cell Plating 16 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress NTSR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0280E2CP0M)
The PathHunter NTSR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neurotensin) based activation of NTSR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NTSR1 and EA-tagged β-Arrestin-2. Activation of NTSR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Exendin-4 Bioassay Kit, 2-Plate. (93-0300Y2-00029)
The PathHunter Exendin-4 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exendin-4 based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cell Biolabs Inc Radius™ 24-Well Cell Migration Assay Kit, 24 assays
Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The Radius™ 24-Well Cell Migration Assay Kit uses a proprietary 24-well plate to monitor migration of cells. Each plate well contains a 0.68 mm non-toxic, biocompatible hydrogel spot where cells do not attach. Adherent cells are seeded and attach everywhere but the Radius™ Gel coated area. Once firm attachment is achieved, the hydrogel is quickly removed to expose a cell-free region across which cells can migrate. The kit provides qualitative or quantitive results with real-time or endpoint analysis, and it is compatible with most HCS and liquid handling instruments. Radius Cell Migration Assays are also available as well in 48-well, 96-well and 384-well formats, and Radius 24-Well Assay Kits are available with wells precoated with Collagen I, Fibronectin or Laminin to promote adhesion of certain cell types.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser239) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-VASP (Ser239) Detection Kit, Control Lysate
Dojindo Molecular Technologies Inc SOD Assay Kit-WST (500 TESTS) is a colorimetric microplate and low background noise measurement which measures 100% inhibition by SOD.
Superoxide dismutase (SOD) is a crucial antioxidative enzyme, which catalyzes the dismutation of the superoxide anion (O2-) into hydrogen peroxide and molecular oxygen. In order to determine SOD activity, several direct and indirect methods have been developed. Among these methods, the indirect method using nitroblue tetrazolium (NBT) is commonly used due to the convenience and ease to use. However, using the NBT method yields poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. SOD Assay Kit-WST allows for very convenient SOD assays by utilizing Dojindo's highly water-soluble tetrazolium salt, WST-1, producing a water-soluble formazan dye upon reduction with the superoxide anion. The rate of the reduction of WST-1 with O2- is linearly related to the xanthine oxidase (XO) activity depending on SOD. Therefore, the IC50 (50% inhibition activity of SOD or SOD-like materials) can be determined by the colorimetric method.
Eurofins Discoverx PathHunter eXpress ADRB1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0488E2CP0L)
The PathHunter ADRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRB1 and EA-tagged β-Arrestin-2. Activation of ADRB1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.